Finally, biocompatibility analysis with a mammalian cell line showed the negligible cytotoxic effect of the fabricated HBPs.ĭendrimers and hyperbranched polymers (HBPs) form a particularly attractive class of AMPs (33−37) because of their locally concentrated functionalities (38−40) and enhanced interaction with a bacterial membrane. Molecular docking simulations of monomeric molecules with PETase revealed different orientations of the molecules at the active site due to the presence of indole or isatin groups, which could be related to the observed different enzymatic degradation behavior. The incorporation of indole into HBPs as well as small molecules facilitated their enzymatic degradation with PETase from Ideonella sakaiensis, while isatin had a complex impact. None of the HBPs leaked out from plastic matrix after being immersed in water for 5 days. HBPs with isatin or methylindole were completely immiscible with the same matrices. According to DSC measurements, up to 20% indole-based HBP is miscible with biodegradable polyesters (polyhydroxybutyrate or polycaprolactone), which can be attributed to the favorable hydrogen bonding between the N–H moiety of indole and the C═O of polyesters. Antimicrobial disk diffusion assay indicated that these HBPs showed significant antibacterial activity against 8 human pathogenic bacteria compared to small molecules with indole or isatin groups.
In this context, nonionic hyperbranched polyesters (HBPs) with indole or isatin functionality were rationally designed, synthesized, and characterized. Most macromolecular antimicrobials are ionic and thus lack miscibility/compatibility with nonionic substrate materials.
0 kommentar(er)
